Nucleocounter Nc 3000

Perform 8 cell viability counts in less than 3 minutes!.

Nucleocounter nc 3000. The NC-0™ PQ Test Kit Via2-Cassettes™ is designed to validate the functionality of the NucleoCounter® NC-0™ when using Via2-Cassettes™. The NucleoCounter® NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells. The NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells.

NucleoView, a software that is the user interface for the NucleoCounter NC-0, NC-250, and NC-3000. Cells were stained with Hoechst-, Annexin V FITC conjugate and Propidium Iodide (PI) and analysed using the Annexin V Assay and a NucleoCounter® NC-3000™. Optional software for the NucleoCounter® NC-100™ family.

The Instruments segment sells NucleoCounter NC-100, NC-0, NC250, and NC-3000. The NucleoCounter ® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NucleoCounter ® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties.

Also used with the NC-0™, NC-250™ and NC-3000™ for “Count of Aggregated Cells A100 and B Assay”. NucleoCounter NC-3000 – Advan. This counting method is typically performed on cells grown on micro carriers.

Detection of DNA fragmentation for late apoptosis evaluation using NucleoCounter® NC-3000™ During apoptosis, nicks and double-strand breaks in the DNA will cause fragmentation of DNA. The ChemoMetec NucleoCounter NC-3000 Advanced Image Cytometer offers your lab flexibility and precise dependability with this all-in-one instrument. GMP Validation and 21 CFR part 11-ready.

GFP transfection efficiency assay using Hoechst and propidium iodide:. Diverse Data Export options The NucleoView™ software provides the panel with different export options, in order to cover the needs of diverse users groups. NucleoCounter® NC-3000™ NucleoCounter® NC-0™.

This late event of apoptosis is detected using DNA content analysis to measure cell having less than one DNA equivalent (so-called sub-G1 cells). Chemometec NucleoCounter NC 3000 Cytometer available from The Lab World Group. Covering early to late stages of apoptosis, these assays will allow for detailed study of progress of apoptosis in mammalian cells.

NucleoCounter Mammalian Cell Counter Sperm Cell Counter Yeast Cell Counter Somatic Cell Counter & More. The NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells. The NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells.

Platelets will not be counted as they will only be very weakly stained. NucleoCounter® NC-3000™ NucleoCounter® NC-0™ NucleoCounter® NC-100™ NucleoCounter® SP-100™ NucleoCounter® YC-100™ Consumables;. The NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells.

The NucleoCounter® SP-100™ Sperm Cell Counter offers unique ease of use and effecti. In normal viability analysis the sample volume is as high as 1.4 µl and the ability to detect cells in clusters is also available. Product Description The NucleoCounter® NC-3000™ test kit 1 consists of one bottle of OQ test beads type 1, one pack of Via1-Cassettes™ (10 cassettes) and one box of NC-slide ™ (25 slides) to verify the functionality of the NC-3000™, also an OQ test plan containing information about the tests to be performed on the instrument.

The NucleoCounter® NC-3000™ enables two assays for measuring GFP transfection efficiency;. NucleoCounter SP-100 – Sperm. Research, quality control or monitoring of production.

The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NucleoCounter® NC-0™ is improved in relation to NucleoCounter® NC-100 and shares some of the strong features with the NucleoCounter® NC-3000. The μFAT samples used in the study had a mean cell.

The Consumer Goods segment offers disposable cartridges, disposable analysis chambers, reagents, and test kits. The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. Type 1 is the least sensitive and should be used for the counting of mammalian cells.

Reagent A100 and B are developed for the NC-100™ where these reagents are used for total cell count. Caspase assay using the Fluorochrome-labeled inhibitor of caspases assay (FLICA) FAM-DEVD-FMK (caspase 3/7), FAM-LETD-FMK (caspase 8) or FAM- LEHD-FMK (caspase 9) NC-3000™ 994 3021:. Examples of types of application are Two-Step Cell Cycle Assay, Cell Cycle of Fixed Cells Assay, DNA Fragmentation Assay, GFP Transfection Efficiency Assay.

The NucleoCounter® NC-3000™ is an advanced cell analyzer utilizing fluorescence imaging to characterize cell properties. Scatter plots and histograms were obtained from the NucleoView™ NC-3000™ software. NucleoCounter ® NC-3000™ NucleoCounter ® NC-2™ NucleoCounter ® NC-0™ NucleoCounter ® SP-100™ NucleoCounter ® YC-100™ Sales Request Demo Request Quote.

It is suitable for a wide range of cell types such as adherent and suspension cell lines and primary cells. Caspase 3/7, 8 & 9:. A bifunctional mechanism in human physiology and disease.

Nastołowy cytometr obrazowy z wbudowanym mikroskopem fluorescencyjnym, NucleoCounter NC-3000 zapewnia analizę przepływo-podobną, o wysokiej powtarzalności bez konieczności kalibracji i. The number of cells and viability was assessed by NucleoCounter NC-3000 using cell viability staining (Chemometech, Allerod, Denmark) 31. Analysis of the level of cellular thiols using the NucleoCounter ® NC-3000™ system:.

The NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells. The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NucleoCounterNC-3000™ serial number is found on the label affixed to the rear of the instrument.

The NucleoCounter ® NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells. The NucleoCounter NC-100+ has 9 different levels of sensitivity, which can be adjusted to count the different types of cells. The NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells.

Image cytometry with the NucleoCounter® family instruments NC-0™, NC-250™ or NC-3000™ allows for fast, precise and easy viability and cell count determination of leukocytes directly in peripheral blood samples. The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The Via1-Cassette™ used together with the NucleoCounter® NC-3000™ enables fast and reliable determination of cell count and viability in one simple step.

It is suitable for a wide range of cell types. The NucleoCounter ® NC-3000™ is an advanced cell analyzer utilizing fluorescence imaging to characterize cell properties. Mitochondrial potential assay using the NucleoCounter ® NC-3000™ system:.

Sales and ordering information. With user adaptable protocols and specialized assays for counting a wide range of mammalian cells, including adipose-derived stem cells, T cells, aggregated cells and cells on microcarriers, the NucleoCounter® NC-0™ provides an all-in-one solution for cell counting and cell. Featuring improved hardware and the new user-friendly NC-View™ software, we take precision, speed, and data management of cell counting to new levels.

The NC-3000™ Cell Cycle Assays use the nuclear stain, DAPI, to measure DNA content. A rapid method taking less than a minute and an advanced assay with increased sensitivity for the detection of cells with low levels of GFP expression. It also provides Xcyto, a quantitative cell imager to analyze adherent and suspension cells;.

The NucleoCounter® NC-3000™ is an advanced cell analyzer utilizing fluorescence imaging to characterize cell properties. The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence Read More » Recent Posts Nonsense-mediated mRNA decay (NMD):. The NucleoCounter ® NC-2™ automated cell counter is our 3 rd generation cassette-based instrument.

The NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells. Mitochondrial Potential Assay, Annexin V Assay, Caspase 3/7, 8 & 9, Cell Vitality Assay and DNA fragmentation Assay. The NucleoCounter® NC-3000™ has 5 plug-and-play apoptosis assays:.

Any researcher who works in a cell culture hood needs some sort of cell counting solution, whether to determine cell concentration prior to cell passage, or to assess cell viability following drug treatment. FlexiCyte™ Protocol for the NucleoCounter® NC-3000™ system:. The NucleoCounter NC-100 is very simple to operate, and only limited training is required.

NC-3000™ Viability and Cell Count Assay – For easy, fast and objective cell counting The Via1-Cassette™ used together with the NucleoCounter® NC- 3000™ enables fast and reliable determination of cell count and vi - ability in one simple step. The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells.

This is a prerequisite for other dyes commonly used for measurement of cell cycle phases, such as propidium iodide. The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence i. Annexin V Assay using the NucleoCounter ® NC-3000™ System:.

The NucleoCounter NC-3000 is a full-spectrum fluorescence microscope which provides precise automated detection, quantification and analysis of fluorescence at the single cell level. The NucleoCounter NC-100+ is very simple to operate, and only limited training is required. DAPI binds specifically to double-stranded DNA and, accordingly, there is no requirement for removing RNA prior to DNA content measurement.

This advanced image cytometer fits on a benchtop, and is ideal for labs specializing in analysis of mammalian, yeast, avian and insect cells thanks to its highspeed cell count and viability determination. The Nuceloview™ data from the NucleoCounter ® NC-250™ and NucleoCounter ® NC-3000™ can be exported to other 3 rd party software, such as FlowJo ®, to handle flow cytometric data. NucleoCounter ® NC-3000™ No rinsing, clogging or maintenance High-speed cell counting and advanced cell analysis Visual inspection of cells – plot to image Build your own assays with up to 4 analytical channels >> Read more.

Cell counters, as the name implies, are tools for counting live and/or dead cells in a culture. The version number of the NucleoView NC-3000™ software shall also be noted, this can be found on the Help – About menu item in the NucleoView NC-3000™ software. Performance qualification (PQ) of laboratory equipment is an important part of instrument validation, in verifying and documenting that the instrument is working reproducibly within the specified working range and limits.

Nucleocounter NC-100 The NucleoCounter NC-100 is a compact instrument wich fi ts perfectly in any mammalian cell laboratory performing e.g.